C in genomic DNA without DNA cleavage.Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes. DNA interrogation by the CRISPR RNA-guided endonuclease Cas9. Precise and predictable CRISPR chromosomal rearrangements reveal principles of Cas9-mediated nucleotide insertion. Highly parallel profiling of Cas9 variant specificity. Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants.
Diversity, classification and evolution of CRISPR-Cas systems. CRISPR-based technologies for the manipulation of eukaryotic genomes. The biology of CRISPR-Cas: backward and forward. The next generation of CRISPR-Cas technologies and applications. The CRISPR tool kit for genome editing and beyond. Editing the epigenome: technologies for programmable transcription and epigenetic modulation. Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation. High-throughput functional genomics using CRISPR-Cas9. RNA-programmed genome editing in human cells. Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Efficient genome editing in zebrafish using a CRISPR-Cas system. RNA-guided human genome engineering via Cas9. Multiplex genome engineering using CRISPR/Cas systems. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
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